Université de Picardie Jules Verne

Three laboratories of Université de Piacrdie Jules Verne are involved in polysaccharide biosynthesis, extraction, modifications and degradation to oligo and monosaccharides.

Plant Biology and innovation (BIOPI), University Picardie Jules Verne; Amiens, France

Description of BIOPI:

The "Plant Biology & Innovation" Research Unit BIOPI EA3900-UPJV is composed of 29 Full Professors and Assistant Professors, 5 engineers and assistants, and 10-20 PhD students and post-doctoral fellows.

The research activity is focused on the "molecular and biochemical study of cell wall and phenylpropanoid modifications during plant development processes".

Three main approaches are developed:

  • Pectin dynamic: Roles of pectin-modifying enzymes during plant development and in response to biotic and abiotic stresses;
  • Phenylpropanoid metabolism: Analysis of the biosynthetic pathway of flax lignans in connection with other metabolic pathways in response to stress and elicitation by oligosaccharides;
  • Technological research: Study and development of biotechnological processes for metabolites and recombinant proteins by plant root.

Description of the polysaccharide research activities:

  • Plant and carbohydrate biochemistry, characterization of cell wall polysaccharides
  • Production and characterization of oligosaccharides
  • Production and characterization of pectin modifying enzymes, interaction of enzymes with protein and chemical inhibitors"

Main pieces of equipments:

HPAEC-PAD, SEC-MALLS, UFLC, GC-MS, electrophoresis, IEF, thermophoresis

Cytology : Microtom, binocular EZ4, protonic microscope, macroscope, epifluorescence microscope, image analysis station

In vitro culture chamber

Molecular modeling

Contact:
Name: Eric Gontier
Email: eric.gontier@u-picardie.fr
Phone: +33 (0)3 22 82 76 81
Fax: +33 (0)3 22 82 76 48
 

Address:
33 rue Saint Leu
UFR sciences,
F-80039 Amiens Cedex
 

Web site: www.biopi.free.fr

Enzyme and Cell Engineering Laboratory – located in 2 sites (Responsible PrKarstenHaupt)

FRE CNRS 3580 Université de Technologie de Compiègne (UTC), Compiègne, France

FRE CNRS 3580 Université de Picardie Jules Verne (UPJV) – Amiens, France (Responsible Pr Catherine Sarazin)

 

Description of GEC FRE CNRS 3580 :

The research developed in GEC laboratory "Enzyme and Cell Engineering" focuses on the study and the implementation of biocatalysts. The common scientific aim among all the investigations consists in creating new functions not existing in nature to explain actual life properties and to produce new tools useful for Biotechnology.

Description of the polysaccharide research activities:

Gec and more precisely the team of professor Catherine Sarazin presents a solid expertise related to the implementation of ionic liquid for (ligno)cellulose pretreatment or biocatalytic approaches (cellulase-catalyzed saccharification in ionic liquid-aqueous systems, lipase-catalyzed acylation in non-conventionnal media, microbial fermentation in presence of ionic liquid) to valorize cellulosic fractions, and other biopolymers (lignin) and biomolecules issued from agro-resources. GEC proposes also transversal approaches to understand the link between enzymatic saccharification of cellulose fraction and structural properties.GEC presents complementary skills in spectroscopic analyses, separative and quantitative techniques, light scattering techniques and scanning electron microscopy. 

Main pieces of equipments:
liquidand solid state NMR, FTIR, Scanning Electron microscopy, HPLC (detectors: UV-visible, refractometer, ELS, PAD), GC, SEC-MALLS, AF4, DLS
 

Contact:
Name: PR Catherine Sarazin

Email: catherine.sarazin@u-picardie.fr

Phone: +33 (0)3 22 82 75 95
Fax: +33 (0)3 22 82 75 95

 
Address:
Unité de Génie Enzymatique et Cellulaire FRE CNRS 3580

Université de Picardie Jules Verne – UFR Sciences

33 rue Saint Leu

80039 Amiens Cedex 1

France
 

Web site: http://www.utc.fr/umr6022/

 

Laboratoire de glycochimie des Antimicrobiens et des Agroressources (LG2A), University Picardie Jules Verne; Amiens, France

The LG2A accounts for 31 permanent members (26 researchers and teaching-researchers, 5 technical and administrative agents) and about 25 contractuals (PhD, post-doc). The research activity involves 2 Axis: (1) Life Chemistry: antivirals, antibacterials, antimalaria, sugar-lectin interactions (analogues, multivalent glycoclusters), vectorisation agents; (2) Sustainable Chemistry: development of methodologies for the modification of carbohydrate substrates from biomass (microwaves, solvent-free reactions, ball-milling). 

Our expertise is on organic and carbohydrate chemistry:

  1. the production of oligosaccharides:
  •  from polysaccharides, by chemical or enzymatic depolymerisation followed by chromatography fractionation (HPLC, HPAEC, LC-MS); [5]
  • from monosaccharides, by regio- and stereoselective synthesis. [6]
  1. the chemical modification of oligosaccharides, by regioselective functionalization.
  2. the complete characterization of the resulting modified oligosaccharides: UV-Vis, FTIR, NMR, HRMS (ESI, MALDI).

 

Main pieces of equipments:

Organic synthesis equipments

Flash chromatography

HPLC (UV, Evaporative Light Scattering Detector), LC-ESI MS, Ionic chromatography

nanoITC

Contact:
Name: José Kovensky
Email: jose.kovensky@u-picardie.fr
Phone: +33 (0)3 22 82 75 67
Fax: +33 (0)3 22 82 75 60

Address:
33 rue Saint Leu
UFR sciences,
F-80039 Amiens Cedex

Website: www.u-picardie.fr/labo/LG/